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Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

《环境科学与工程前沿（英文）》 2020年第14卷第6期 doi: 10.1007/s11783-020-1274-5

摘要： Abstract • Sampling parameters with high efficiency was determined. • Operational process to detect airborne ARGs was optimized. • Providing research basis to control airborne ARGs of a laboratory atmosphere Antibiotic resistance genes (ARGs) have been detected in various atmospheric environments. Airborne ARGs transmission presents the public health threat. However, it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples. In this study, an optimized protocol for collecting and detecting airborne ARGs was presented. Experimental results showed that recovery efficiency tended to increase initially and then declined over time, and a range of 550–780 copies/mm2 of capture loading was recommended to ensure that the recovery efficiency is greater than 75%. As the cell walls were mechanically disrupted and nucleic acids were released, the buffer wash protects ARGs dissolution. Three ratios of buffer volume to membrane area in buffer wash were compared. The highest concentrations of airborne ARGs were detected with 1.4 µL/mm2 buffer wash. Furthermore, the majority of the cells were disrupted by an ultrasonication pretreatment (5 min), allowing the efficiency ARGs detection of airborne samples. While, extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments. Therefore, this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments. An optimized sampling method was proposed that the buffer wash was 1.4 µL/mm2 and the ultrasonication duration was 5 min. The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories. The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

关键词： Airborne tetracycline resistance genes Filter sampling Capture loading Membrane pretreatment Indoor laboratory atmosphere

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Bioengineering protection mechanism of city rock slope and its laboratory test

Desong JIANG, Minghua ZHAO, Chong JIANG,

《结构与土木工程前沿（英文）》 2009年第3卷第4期页码 414-421 doi: 10.1007/s11709-009-0055-9

摘要： Based on the features of rock slope bioengineering protection, the ecology protection mechanism of the urban rock slope was discussed with the mechanics effect of plants and rock slope, and the reinforcement action mechanism of rock slope by plant root system was analyzed as well. Then, the corresponding mechanical model was proposed, from which the formula to calculate the increased shearing strength of the root system-earth compound body was derived. Moreover, the side slope rainfall interception, the runoff lagging, the soil antiseepage, and the soil layer consolidating effect were studied, respectively. Furthermore, the indoor model experiment of urban crag rock slope ecology protection was designed and completed, in which various grasses to plant in slope with different angles, solid earth forms, and the different strengthening earth mechanism were studied. Finally, the present method was applied in an engineering project, from which the antiwashing behavior of three kind of grasses (i.e., the Bahiagrass, the tall fescue, and the Bermudagrass) planted in the slope with an angle of 38°, 48°, and 58°, respectively, and different strengthening structures (i.e., the diamond wire netting, the geocell and the three-dimensional network) were obtained. The application results also show that the effect of geocell structure is the best one followed by the three-dimensional net and the diamond wire net. The antiwashing capability per unit area has a critical slope angle of about 25°. The reinforcing effect of Bermudagrass is better than the Bahiagrass and tall fescue.

关键词： indoor engineering project protection mechanism different runoff lagging

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Digital twin for healthy indoor environment: A vision for the post-pandemic era

《工程管理前沿（英文）》 2023年第10卷第2期页码 300-318 doi: 10.1007/s42524-022-0244-y

摘要： Indoor environment has significant impacts on human health as people spend 90% of their time indoors. The COVID-19 pandemic and the increased public health awareness have further elevated the urgency for cultivating and maintaining a healthy indoor environment. The advancement in emerging digital twin technologies including building information modeling (BIM), Internet of Things (IoT), data analytics, and smart control have led to new opportunities for building design and operation. Despite the numerous studies on developing methods for creating digital twins and enabling new functionalities and services in smart building management, very few have focused on the health of indoor environment. There is a critical need for understanding and envisaging how digital twin paradigms can be geared towards healthy indoor environment. Therefore, this study reviews the techniques for developing digital twins and discusses how the techniques can be customized to contribute to public health. Specifically, the current applications of BIM, IoT sensing, data analytics, and smart building control technologies for building digital twins are reviewed, and the knowledge gaps and limitations are discussed to guide future research for improving environmental and occupant health. Moreover, this paper elaborates a vision for future research on integrated digital twins for a healthy indoor environment with special considerations of the above four emerging techniques and issues. This review contributes to the body of knowledge by advocating for the consideration of health in digital twin modeling and smart building services and presenting the research roadmap for digital twin-enabled healthy indoor environment.

关键词： digital twin healthy indoor environment building information modeling occupant–building interaction COVID-19

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Optimized porous clay heterostructure for removal of acetaldehyde and toluene from indoor air

Pu ZHAO,Lizhong ZHU

《环境科学与工程前沿（英文）》 2016年第10卷第2期页码 219-228 doi: 10.1007/s11783-014-0760-z

摘要： Adsorption is the most widely used technology for the removal of indoor volatile organic compounds (VOCs). However, existing adsorbent-based technologies are inadequate to meet the regulatory requirement, due to their limited adsorption capacity and efficiency, especially under high relative humidity (RH) conditions. In this study, a series of new porous clay heterostructure (PCH) adsorbents with various ratios of micropores to mesopores were synthesized, characterized and tested for the adsorption of acetaldehyde and toluene. Two of them, PCH25 and PCH50, exhibited markedly improved adsorption capability, especially for hydrophilic acetaldehyde. The improved adsorption was attributed to their large micropore areas and high micropore-to-mesopore volume ratios. The amount of acetaldehyde adsorbed onto PCH25 at equilibrium reached 62.7 mg·g , eight times as much as the amount adsorbed onto conventional activated carbon (AC). Even at a high RH of 80%, PCH25 removed seven and four times more of the acetaldehyde than AC and the unmodified raw PCHs did, respectively. This new PCH optimized for their high adsorption and resistance to humidity has promising applications as a cost-effective adsorbent for indoor air purification.

关键词： porous clay heterostructure volatile organic compounds adsorption adsorbent indoor air

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Comparative analysis of impact of human occupancy on indoor microbiomes

《环境科学与工程前沿（英文）》 2021年第15卷第5期 doi: 10.1007/s11783-020-1383-1

摘要：

• Exposure to indoor microbiomes is a public health concern in educational facilities.

关键词： Built environment Indoor microbiome Occupant Building Sequencing

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Relations between indoor and outdoor PM

Cong Liu, Yinping Zhang

《环境科学与工程前沿（英文）》 2019年第13卷第1期 doi: 10.1007/s11783-019-1089-4

摘要：

Factors impacting indoor-outdoor relations are introduced.

Sulfate seems a fine tracer for other non-volatile species.

Particulate nitrate and ammonium desorb during outdoor-to-indoor transport.

OC load increases during the transport due to sorption of indoor SVOCs.

关键词： Indoor air quality Exposure SVOC Reactive oxidative species Oxidative potential Chemical transport model

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Indoor carbonyl compounds in an academic building in Beijing, China: concentrations and influencing factors

Chuanjia JIANG, Pengyi ZHANG

《环境科学与工程前沿（英文）》 2012年第6卷第2期页码 184-194 doi: 10.1007/s11783-011-0309-3

摘要： Carbonyl compounds in indoor air are of great concern for their adverse health effects. Between February and May, 2009, concentrations of 13 carbonyl compounds were measured in an academic building in Beijing, China. Total concentration of the detected carbonyls ranged from 20.7 to 189.1 μg·m , and among them acetone and formaldehyde were the most abundant, with mean concentrations of 26.4 and 22.6 μg·m , respectively. Average indoor concentrations of other carbonyls were below 10 μg·m . Principal component analysis identified a combined effect of common indoor carbonyl sources and ventilation on indoor carbonyl levels. Diurnal variations of the carbonyl compounds were investigated in one office room, and carbonyl concentrations tended to be lower in the daytime than at night, due to enhanced ventilation. Average concentrations of carbonyl compounds in the office room were generally higher in early May than in late February, indicating the influence of temperature. Carbonyl source emission rates from both the room and human occupants were estimated during two lectures, based on one-compartment mass balance model. The influence of human occupants on indoor carbonyl concentrations varies with environmental conditions, and may become significant in the case of a large human occupancy.

关键词： carbonyl compounds indoor air ventilation human occupancy source emission rate (SER) principal component analysis (PCA)

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Laboratory diagnosis for malaria in the elimination phase in China: efforts and challenges

《医学前沿（英文）》 2022年第16卷第1期页码 10-16 doi: 10.1007/s11684-021-0889-7

摘要： Malaria remains a global health challenge, although an increasing number of countries will enter pre-elimination and elimination stages. The prompt and precise diagnosis of symptomatic and asymptomatic carriers of Plasmodium parasites is the key aspect of malaria elimination. Since the launch of the China Malaria Elimination Action Plan in 2010, China has formulated clear goals for malaria diagnosis and has established a network of malaria diagnostic laboratories within medical and health institutions at all levels. Various external quality assessments were implemented, and a national malaria diagnosis reference laboratory network was established to strengthen the quality assurance in malaria diagnosis. Notably, no indigenous malaria cases have been reported since 2017, but the risk of re-establishment of malaria transmission cannot be ignored. This review summarizes the lessons about malaria diagnosis in the elimination phase, primarily including the establishments of laboratory networks and quality control in China, to better improve malaria diagnosis and maintain a malaria-free status. A reference is also provided for countries experiencing malaria elimination.

关键词： malaria laboratory diagnosis quality control malaria elimination China

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Erratum to: Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

《环境科学与工程前沿（英文）》 2020年第14卷第6期 doi: 10.1007/s11783-020-1289-y

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Effect of natural resource on improving indoor thermal environment in Chongqing

Yong DING, Baizhan LI, Qing LUO, Hong LIU, Meng LIU

《结构与土木工程前沿（英文）》 2009年第3卷第2期页码 211-218 doi: 10.1007/s11709-009-0018-1

摘要： This paper analyzes the potential of natural resources to improve the indoor thermal environment in Chongqing through the statistical analysis of natural resources including solar energy, wind, water, and earth, etc. The building form, systems, and principle of usage of natural resources are briefly analyzed through the building site decision, building form design, and computer simulation, which will be the real reference for the design of building energy efficiency.

关键词： natural resource indoor thermal environment renewable energy

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Evaluation of computer vision techniques for automated hardhat detection in indoor construction safety

Bahaa Eddine MNEYMNEH, Mohamad ABBAS, Hiam KHOURY

《工程管理前沿（英文）》 2018年第5卷第2期页码 227-239 doi: 10.15302/J-FEM-2018071

摘要： Construction is considered among the most dangerous industries and is responsible for a large portion of total worker fatalities. A construction worker has a probability of 1-in-200 of dying on the job during a 45-year career, mainly due to fires, falls, and being struck by or caught between objects. Hence, employers must ensure their workers wear personal protective equipment (PPE), in particular hardhats, if they are at risk of falling, being struck by falling objects, hitting their heads on static objects, or coming in proximity to electrical hazards. However, monitoring the presence and proper use of hardhats becomes inefficient when safety officers must survey large areas and a considerable number of workers. Using images captured from indoor jobsites, this paper evaluates existing computer vision techniques, namely object detection and color-based segmentation tools, used to rapidly detect if workers are wearing hardhats. Experiments are conducted and the results highlight the potential of cascade classifiers, in particular, to accurately, precisely, and rapidly detect hardhats under different scenarios and for repetitive runs, and the potential of color-based segmentation to eliminate false detections.

关键词： construction safety personal protective equipment hardhat computer vision

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Formation of secondary inorganic aerosol in a frigid urban atmosphere

《环境科学与工程前沿（英文）》 2022年第16卷第2期 doi: 10.1007/s11783-021-1452-0

摘要：

•Harbin showed relatively high threshold RH (80%) for apparent increase of SOR.

关键词： Haze Sulfate Nitrate Heterogeneous chemistry Biomass burning Northeast China

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Bridging the gap between laboratory and field moduli of asphalt layer for pavement design and assessment

Huailei CHENG; Liping LIU; Lijun SUN

《结构与土木工程前沿（英文）》 2022年第16卷第3期页码 267-280 doi: 10.1007/s11709-022-0811-7

摘要： Asphalt pavement is a key component of highway infrastructures in China and worldwide. In asphalt pavement design and condition assessment, the modulus of the asphalt mixture layer is a crucial parameter. However, this parameter varies between the laboratory and field loading modes (i.e., loading frequency, compressive or tensile loading pattern), due to the viscoelastic property and composite structure of the asphalt mixture. The present study proposes a comprehensive frequency-based approach to correlate the asphalt layer moduli obtained under two field and three laboratory loading modes. The field modes are vehicular and falling weight deflectometer (FWD) loading modes, and the laboratory ones are uniaxial compressive (UC), indirect tensile (IDT), and four-point bending (4PB) loading modes. The loading frequency is used as an intermediary parameter for correlating the asphalt layer moduli under different loading modes. The observations made at two field large-scale experimental pavements facilitate the correlation analysis. It is found that the moduli obtained via laboratory 4PB tests are pretty close to those of vehicular loading schemes, in contrast to those derived in UC, IDT, and FWD modes, which need to be adjusted. The corresponding adjustment factors are experimentally assessed. The applications of those adjustment factors are expected to ensure that the moduli measured under different loading modes are appropriately used in asphalt mixture pavement design and assessment.

关键词： asphalt mixture layer stiffness modulus loading mode UC/4PB/IDT FWD frequency

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Numerical and experimental analyses of methane leakage in shield tunnel

《结构与土木工程前沿（英文）》页码 1011-1020 doi: 10.1007/s11709-023-0956-z

摘要： Tunnels constructed in gas-bearing strata are affected by the potential leakage of harmful gases, such as methane gas. Based on the basic principles of computational fluid dynamics, a numerical analysis was performed to simulate the ventilation and diffusion of harmful gases in a shield tunnel, and the effect of ventilation airflow speed on the diffusion of harmful gases was evaluated. As the airflow speed increased from 1.8 to 5.4 m/s, the methane emission was diluted, and the methane accumulation was only observed in the area near the methane leakage channels. The influence of increased ventilation airflow velocity was dominant for the ventilation modes with two and four fans. In addition, laboratory tests on methane leakage through segment joints were performed. The results show that the leakage process can be divided into “rapid leakage” and “slight leakage”, depending on the leakage pressure and the state of joint deformation. Based on the numerical and experimental analysis results, a relationship between the safety level and the joint deformation is established, which can be used as guidelines for maintaining utility tunnels.

关键词： shield tunnel harmful gas leakage numerical analysis laboratory test

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Reduction of CeO

Elena Yu. KONYSHEVA

《化学科学与工程前沿（英文）》 2013年第7卷第3期页码 249-261 doi: 10.1007/s11705-013-1333-6

摘要： Reduction behavior of pure and doped CeO , the multi-phase La Sr CoO ? CeO , La Sr MnO ? CeO , and La Ni Fe O ? CeO composites, was studied under hydrogen containing atmosphere to address issues related to the improvement of electrochemical and catalytic performance of electrodes in fuel cells. The enhanced reduction of cerium oxide was observed initially at 800°C in all composites in spite of the presence of highly reducible transition metal cations that could lead to the increase in surface concentration of oxygen vacancies and generation of the electron enriched surface. Due to continuous reduction of cerium oxide in La Sr CoO ? CeO and La Sr MnO ? CeO (up to 10 h) composites the redox activity of the Ce /Ce pair could be suppressed and additional measures are required for reversible spontaneous regeneration of Ce . After 3 h exposure to H -Ar at 800°C the reduction of cerium oxides and perovskite phases in La Ni Fe O ? CeO composites was diminished. The extent of cerium oxide involvement in the reduction process varies with time, and depends on its initial deviation from oxygen stoichiometry (that results in the larger lattice parameter and the longer pathway for O transport through the fluorite lattice), chemical origin of transition metal cations in the perovskite, and phase diversity in multi-phase composites.

关键词： reduction of cerium oxide composites perovskites catalyst under hydrogen containing atmosphere